Wentworth D E, McGregor M W, Macklin M D, Neumann V, Hinshaw V S
Department of Pathobiological Sciences, University of Wisconsin-Madison, USA.
J Infect Dis. 1997 Jan;175(1):7-15. doi: 10.1093/infdis/175.1.7.
Two people developed symptoms of influenza 36 h after collecting nasal swabs from pigs experimentally infected with A/Sw/IN/1726/88 (Sw/IN). Pharyngeal swabs from these persons tested positive for influenza virus RNA 8 days after infection. Analysis of hemi-nested polymerase chain reaction (PCR) products indicated that the hemagglutinin (HA) segments of the isolates were genetically related to the HA of Sw/IN. Four influenza A virus isolates (A/WI/4754/94, A/WI/4756/94, A/WI/4758/94, A/WI/4760/94) were recovered from a 39-year-old man and 2 (A/WI/4755/94, A/WI/4757/94) from a 31-year-old woman. The HAs of the isolates were antigenically indistinguishable from the virus used to infect the pigs. Sequence analysis of the HA genes indicated they were 99.7% identical to the HA of the virus used in the experiment. Multisegment reverse transcription-PCR proved that all of the segments originated from Sw/IN, demonstrating that transmission of swine H1N1 viruses to humans occurs directly and readily, despite Animal Biosafety Level 3 containment practices used for these experiments.
在从实验感染A/Sw/IN/1726/88（Sw/IN）的猪身上采集鼻拭子36小时后，有两人出现流感症状。感染8天后，这些人的咽拭子流感病毒RNA检测呈阳性。对半巢式聚合酶链反应（PCR）产物的分析表明，分离株的血凝素（HA）片段在基因上与Sw/IN的HA相关。从一名39岁男子身上分离出4株甲型流感病毒（A/WI/4754/94、A/WI/4756/94、A/WI/4758/94、A/WI/4760/94），从一名31岁女子身上分离出2株（A/WI/4755/94、A/WI/4757/94）。分离株的HA在抗原性上与用于感染猪的病毒无法区分。HA基因的序列分析表明，它们与实验中使用的病毒的HA有99.7%的同一性。多片段逆转录PCR证明所有片段均源自Sw/IN，这表明猪H1N1病毒可直接且容易地传播给人类，尽管这些实验采用了动物生物安全3级防护措施。
