Rikihisa Y, Zhi N, Wormser G P, Wen B, Horowitz H W, Hechemy K E
Department of Veterinary Biosciences, College of Veterinary Medicine, Ohio State University, Columbus 43210-1093, USA.
J Infect Dis. 1997 Jan;175(1):210-3. doi: 10.1093/infdis/175.1.210.
A human granulocytic ehrlichiosis (HGE) agent with 16S rDNA sequence identical to the published sequence of HGE agents was isolated from a patient from New York State by inoculation of the blood leukocyte fraction directly into a human promyelocytic leukemia cell line HL-60. The HGE agent was also isolated from the leukocyte fraction of the blood and bone marrow of a mouse inoculated with the leukocyte fraction of the patient's blood. The isolate has been passaged in tissue culture 30 times over 8 months. Electron microscopy revealed pleomorphic coccobacilli with a thin and highly rippled outer membrane in the clear inclusion matrix. Comparison of IFA reactivity of antisera obtained from a variety of sources with the cell-cultured HGE agent revealed that 3 HGE agent strains (New York isolate, Wisconsin [BDS] isolate, and a tick-derived isolate) are highly cross-reactive and there are diverse antigenic cross-reactivities between HGE agent and Ehrlichia chaffeensis.
通过将血液白细胞组分直接接种到人类早幼粒细胞白血病细胞系HL-60中，从一名纽约州患者体内分离出一种16S rDNA序列与已发表的人粒细胞埃立克体病（HGE）病原体序列相同的HGE病原体。该HGE病原体也从接种了患者血液白细胞组分的小鼠的血液和骨髓白细胞组分中分离得到。该分离株已在组织培养中传代30次，历时8个月。电子显微镜检查显示，在透明包涵体基质中有多形性球杆菌，其外膜薄且高度起伏。对从多种来源获得的抗血清与细胞培养的HGE病原体的免疫荧光分析（IFA）反应性进行比较，结果显示3种HGE病原体菌株（纽约分离株、威斯康星州[BDS]分离株和一种蜱源分离株）具有高度交叉反应性，并且HGE病原体与恰菲埃立克体之间存在多种抗原交叉反应性。
