Ostergaard M, Pedersen L, Schmidt J, Luz A, Lovmand J, Erfle V, Pedersen F S, Strauss P G
Department of Molecular and Structural Biology, University of Aarhus, Denmark.
J Virol. 1997 Jan;71(1):645-9. doi: 10.1128/JVI.71.1.645-649.1997.
Certain isolates of murine leukemia viruses (MuLVs) have, apart from a leukemogenic potential, the capability of inducing diseases of nonhematopoietic tissues in susceptible strains of mice. We have reported on the molecular cloning of a bone-tumorigenic virus, RFB-14 MuLV, which was found to induce benign bone tumors, osteomas, with 100% incidence in mice of the CBA/Ca strain (L. Pedersen, W. Behnisch, J. Schmidt, A. Luz, F. S. Pedersen, V. Erfle, and P. G. Strauss, J. Virol. 66:6186-6190, 1992). In order to analyze the bone tumor-inducing phenotype of RFB-14 MuLV, we have studied the pathogenic potential of recombinant viruses between RFB-14 and the nonosteomagenic, highly leukemogenic SL3-3 MuLV. The recombinants were constructed so as to reveal whether a major determinant of osteomagenicity maps to sequences within or outside the long terminal repeats (LTR). Our data show that a major determinant of the osteoma-inducing potential of RFB-14 MuLV maps to the non-LTR region of the genome. Furthermore, we demonstrate that a strong determinant of leukemogenicity is harbored by the non-LTR region of SL3-3 MuLV.
某些鼠白血病病毒（MuLVs）分离株，除了具有致白血病潜力外，还能够在易感小鼠品系中诱发非造血组织疾病。我们报道了一种骨致瘤病毒RFB - 14 MuLV的分子克隆，该病毒在CBA/Ca品系小鼠中可100%诱发良性骨肿瘤——骨瘤（L. 佩德森、W. 贝尼施、J. 施密特、A. 卢兹、F. S. 佩德森、V. 埃尔弗勒和P. G. 施特劳斯，《病毒学杂志》66:6186 - 6190，1992年）。为了分析RFB - 14 MuLV的骨肿瘤诱导表型，我们研究了RFB - 14与非骨致瘤、高致白血病性的SL3 - 3 MuLV之间重组病毒的致病潜力。构建重组病毒是为了揭示骨致瘤性的主要决定因素是否定位于长末端重复序列（LTR）内部或外部的序列。我们的数据表明，RFB - 14 MuLV骨瘤诱导潜力的主要决定因素定位于基因组的非LTR区域。此外，我们证明SL3 - 3 MuLV的非LTR区域含有致白血病性的强决定因素。
