Tribet C, Audebert R, Popot J L
Centre National de la Recherche Scientifique Unité de Recherche Associée 278, Paris, France.
Proc Natl Acad Sci U S A. 1996 Dec 24;93(26):15047-50. doi: 10.1073/pnas.93.26.15047.
Amphipols are a new class of surfactants that make it possible to handle membrane proteins in detergent-free aqueous solution as though they were soluble proteins. The strongly hydrophilic backbone of these polymers is grafted with hydrophobic chains, making them amphiphilic. Amphipols are able to stabilize in aqueous solution under their native state four well-characterized integral membrane proteins: (i) bacteriorhodopsin, (ii) a bacterial photosynthetic reaction center, (iii) cytochrome b6f, and (iv) matrix porin.
双亲分子是一类新型表面活性剂，它使得在无去污剂的水溶液中处理膜蛋白成为可能，就好像它们是可溶性蛋白一样。这些聚合物具有强亲水性主链，并接枝有疏水链，使其具有两亲性。双亲分子能够在水溶液中使其天然状态下的四种特征明确的整合膜蛋白保持稳定：（i）细菌视紫红质，（ii）细菌光合反应中心，（iii）细胞色素b6f，以及（iv）基质孔蛋白。
