Tribet C, Mills D, Haider M, Popot J L
CNRS-UMR 7615, Paris, France.
Biochimie. 1998 May-Jun;80(5-6):475-82. doi: 10.1016/s0300-9084(00)80014-0.
The composition and mass of complexes between Chlamydomonas reinhardtii cytochrome b6f and low molecular mass amphipathic polymers ('amphipols') have been studied using biochemical analysis and scanning transmission electron microscopy at liquid helium temperature (cryo-STEM). Cytochrome b6f was trapped by amphipols either under its native 14-meric state or as a delipidated, lighter form. A good consistency was observed between the masses of either form calculated from their biochemical composition and those determined by cryo-STEM. These data show that association with amphipols preserved the original original state of the protein in detergent solution. Complexation with amphipols appears to facilitate preparation of the samples and mass determination by cryo-STEM as compared to conventional solubilization with detergents.
利用生化分析和液氦温度下的扫描透射电子显微镜（低温扫描透射电子显微镜，cryo-STEM），对莱茵衣藻细胞色素b6f与低分子量两亲聚合物（“两性离子聚合物”）之间复合物的组成和质量进行了研究。细胞色素b6f在其天然的十四聚体状态下或作为脱脂的较轻形式被两性离子聚合物捕获。从生化组成计算得到的两种形式的质量与通过低温扫描透射电子显微镜测定的质量之间观察到了良好的一致性。这些数据表明，与两性离子聚合物结合可使蛋白质在去污剂溶液中保持原始状态。与用去污剂进行的传统溶解相比，与两性离子聚合物复合似乎有助于样品的制备和通过低温扫描透射电子显微镜进行质量测定。
