Toniolli R, Bussière J, Courot M, Magistrini M, Combarnous Y
Physiologie de la reproduction des mammifères domestiques, Inra-CNRS URA1291, Nouzilly, France.
Reprod Nutr Dev. 1996;36(5):503-11. doi: 10.1051/rnd:19960506.
In order to extend the duration of boar sperm survival at 15 degrees C for artificial insemination, we tested the effect of indole-3-acetic acid (IAA), which appeared to be the main sperm protective substance present in the Coco nucifera endosperm (coconut water). Two IAA concentrations (10 and 100 ng/mL) in Beltsville extender (BTS) were studied for their in vitro effects. The motility, the percentage of motile spermatozoa and the acrosome morphology of sperm were recorded each day over 13 days of storage at 15 degrees C, after 5 min and 3 h of incubation at 39 degrees C. The IAA effect on sperm preservation was also studied in vivo at a concentration of 10 ng/mL in BTS by inseminating groups of females twice at 24 h intervals either at D0 (day of sperm collection) and D1 (D0/1) or at D5 and D6 (D5/6). At D0/1, the two groups of females (control and IAA) were inseminated with a total of 6.3 x 10(9) spermatozoa (3.15 x 10(9) at D0 and the same dose at D1) whereas at D5/6, on IAA group was inseminated with a total of 6.3 x 10(9) spermatozoa and another one with 12.6 x 10(9) spermatozoa. The animals in the D5/6 control group were inseminated each with a total of 12.6 x 10(9) spermatozoa. For each group of females (n = 106-140), fertility rate (% farrowing) and prolificacy rate (litter size) were recorded. No effect of IAA in vitro on the motility rate and on the percentage of motile spermatozoa was observed over a 13 day storage. However, IAA (10 ng/mL) had a significant positive effect on the percentage of living spermatozoa with intact acrosomes after 13 days (66 vs 54%, P < 0.05). The fertility and prolificacy rates after 5-6 days of sperm preservation in BTS extender alone did not differ significantly between D5/6 and D0/1 but the total number of inseminated spermatozoa was 12.6 x 10(9) at D5/6 instead of 6.3 x 10(9) at D0/1. When the spermatozoa were stored in the presence of 10 ng/mL IAA for 5-6 days at 15 degrees C, the fertility and prolificacy of the females inseminated with only 6.3 x 10(9) spermatozoa were identical to those of the females inseminated with an equal number of spermatozoa at D0/1 in the presence or absence of IAA.
为了延长公猪精子在15℃下用于人工授精的存活时间，我们测试了吲哚-3-乙酸（IAA）的作用，它似乎是椰果胚乳（椰子水）中存在的主要精子保护物质。研究了在Beltsville稀释液（BTS）中两种IAA浓度（10和100 ng/mL）的体外效应。在15℃下储存13天期间，每天记录精子的活力、活动精子百分比和顶体形态，记录在39℃下孵育5分钟和3小时后的情况。还通过在D0（采精日）和D1（D0/1）或D5和D6（D5/6）以24小时间隔对雌性组进行两次授精，在体内研究了BTS中10 ng/mL浓度的IAA对精子保存的影响。在D0/1，两组雌性（对照组和IAA组）总共授精6.3×10⁹个精子（D0时为3.15×10⁹个，D1时剂量相同），而在D5/6，一个IAA组总共授精6.3×10⁹个精子，另一个组授精12.6×10⁹个精子。D5/6对照组的动物每头总共授精12.6×10⁹个精子。对于每组雌性（n = 106 - 140），记录生育率（产仔率）和产仔数。在13天的储存期内，未观察到IAA在体外对活力率和活动精子百分比有影响。然而，13天后，IAA（10 ng/mL）对顶体完整的活精子百分比有显著的积极影响（66%对54%，P < 0.05）。仅在BTS稀释液中保存5 - 6天后的生育率和产仔数在D5/6和D0/1之间没有显著差异，但D5/6时授精精子总数为12.6×10⁹个，而D0/1时为6.3×10⁹个。当精子在10 ng/mL IAA存在下于15℃保存5 - 6天时，仅用6.3×10⁹个精子授精的雌性的生育率和产仔数与在D0/1存在或不存在IAA情况下用相同数量精子授精的雌性相同。
