Misra G, Pavlostathis S G, Perdue E M, Araujo R
School of Civil and Environmental Engineering, Georgia Institute of Technology, Atlanta 30332-0512, USA.
Appl Microbiol Biotechnol. 1996 Jul;45(6):831-8. doi: 10.1007/s002530050770.
Batch experiments were conducted to assess the biotransformation potential of four hydrocarbon monoterpenes (d-limonene, alpha-pinene, gamma-terpinene, and terpinolene) and four alcohols (arbanol, linalool, plinol, and alpha-terpineol) under aerobic conditions at 23 degrees C. Both forest-soil extract and enriched cultures were used as inocula for the biodegradation experiments conducted first without, then with prior microbial acclimation to the monoterpenes tested. All four hydrocarbons and two alcohols were readily degraded. The increase in biomass and headspace CO2 concentrations paralleled the depletion of monoterpenes, thus confirming that terpene disappearance was the result of biodegradation accompanied by microbial growth and mineralization. Plinol resisted degradation in assays using inocula from diverse sources, while arbanol degraded very slowly. A significant fraction of d-limonene-derived carbon was accounted for as non-extractable, dissolved organic carbon, whereas terpineol exhibited a much higher degree of utilization. The rate and extent of monoterpene biodegradation were not significantly affected by the presence of dissolved natural organic matter.
进行了批次实验，以评估四种烃类单萜（d-柠檬烯、α-蒎烯、γ-松油烯和萜品油烯）和四种醇类（芳樟醇、芳樟醇、松油醇和α-松油醇）在23摄氏度有氧条件下的生物转化潜力。森林土壤提取物和富集培养物都用作生物降解实验的接种物，这些实验首先在没有预先使微生物适应所测试单萜的情况下进行，然后在有预先适应的情况下进行。所有四种烃类和两种醇类都很容易降解。生物量和顶空二氧化碳浓度的增加与单萜的消耗平行，从而证实萜烯的消失是生物降解伴随着微生物生长和矿化的结果。在使用来自不同来源的接种物的试验中，松油醇抵抗降解，而芳樟醇降解非常缓慢。相当一部分d-柠檬烯衍生的碳被认为是不可提取的溶解有机碳，而松油醇表现出更高的利用率。溶解的天然有机物的存在对单萜生物降解的速率和程度没有显著影响。
