Sales Adones, Afonso Luana Ferreira, Americo Juliana Alves, de Freitas Rebelo Mauro, Pastore Glaucia Maria, Bicas Juliano Lemos
Laboratory of Bioflavors and Bioactive Compounds, Department of Food Science, Faculty of Food Engineering, University of Campinas, Monteiro Lobato street, 80, Campinas, São Paulo, 13083-862, Brazil.
Institute of Biophysics Carlos Chagas Filho, Center for Health Sciences (CCS), Federal University of Rio de Janeiro, Carlos Chagas Filho Avenue, 373, Cidade Universitária - Ilha do Fundão, Rio de Janeiro, RJ, 21941-902, Brazil.
Biotechnol Lett. 2018 Mar;40(3):561-567. doi: 10.1007/s10529-017-2503-2. Epub 2017 Dec 29.
To investigate the biocatalytic potential of Colletotrichum acutatum and Colletotrichum nymphaeae for monoterpene biotransformation.
C. acutatum and C. nymphaeae used limonene, α-pinene, β-pinene, farnesene, citronellol, linalool, geraniol, perillyl alcohol, and carveol as sole carbon and energy sources. Both species biotransformed limonene and linalool, accumulating limonene-1,2-diol and linalool oxides, respectively. α-Pinene was only biotransformed by C. nymphaeae producing campholenic aldehyde, pinanone and verbenone. The biotransformation of limonene by C. nymphaeae yielded 3.34-4.01 g limonene-1,2-diol l, depending on the substrate (R-(+)-limonene, S-(-)-limonene or citrus terpene (an agro-industrial by-product). This is among the highest concentrations already reported for this product.
This is the first report on the biotransformation of these terpenes by Colletotrichum spp. and the biotransformation of limonene to limonene-1,2-diol possibly involves enzymes similar to those found in Grosmannia clavigera.
研究尖孢炭疽菌和睡莲炭疽菌对单萜进行生物转化的潜力。
尖孢炭疽菌和睡莲炭疽菌利用柠檬烯、α-蒎烯、β-蒎烯、法尼烯、香茅醇、芳樟醇、香叶醇、紫苏醇和香芹醇作为唯一的碳源和能源。两种菌株都对柠檬烯和芳樟醇进行了生物转化，分别积累了柠檬烯-1,2-二醇和芳樟醇氧化物。α-蒎烯仅被睡莲炭疽菌生物转化，产生龙脑醛、蒎烷酮和马鞭草烯酮。睡莲炭疽菌对柠檬烯的生物转化产生了3.34 - 4.01 g/L的柠檬烯-1,2-二醇，这取决于底物（R-(+)-柠檬烯、S-(-)-柠檬烯或柑橘萜烯（一种农业工业副产品））。这是该产物已报道的最高浓度之一。
这是关于炭疽菌属对这些萜类进行生物转化的首次报道，柠檬烯向柠檬烯-1,2-二醇的生物转化可能涉及与格氏炭疽菌中发现的类似的酶。
