Pippia P, Sciola L, Cogoli-Greuter M, Meloni M A, Spano A, Cogoli A
Istituto di Fisiologia Generale, University of Sassari, Italy.
J Biotechnol. 1996 Jun 27;47(2-3):215-22. doi: 10.1016/0168-1656(96)01387-9.
Human peripheral blood lymphocytes and monocytes were activated with concanavalin A with or without exogenous recombinant interleukin 1 (IL-1) alone or IL-1 + interleukin 2 (IL-2) under microgravity conditions to test the hypothesis that lack of production of IL-1 by monocytes is the cause of the near total loss of activation observed earlier on several Spacelab flights. The 60 min failure of the on-board 1 x g reference centrifuge at the time of the addition of the activator renders the in-flight data at 1 x g unreliable. However, the data from a previous experiment on SLS-1 show that there is no difference between the results from the in-flight 1 x g centrifuge and 1 x g on ground. The comparison between the data of the cultures at 0 x g in space and of the synchronous control at 1 x g on ground show that exogenous IL-1 and IL-2 do not prevent the loss of activity (measured as the mitotic index) at 0 x g; production of interferon-gamma, however, is partially restored. In contrast to a previous experiment in space, the production of IL-1 is not inhibited.
在微重力条件下，用伴刀豆球蛋白A单独或与外源性重组白细胞介素1（IL-1）或IL-1 +白细胞介素2（IL-2）一起激活人外周血淋巴细胞和单核细胞，以检验单核细胞缺乏IL-1产生是早期在几次航天实验室飞行中观察到的激活近乎完全丧失的原因这一假设。在添加激活剂时，机载1xg参考离心机出现60分钟故障，使得飞行中1xg时的数据不可靠。然而，先前在SLS-1上进行的一项实验的数据表明，飞行中1xg离心机的结果与地面上1xg的结果没有差异。空间中0xg培养物的数据与地面上1xg同步对照的数据比较表明，外源性IL-1和IL-2不能防止0xg时活性（以有丝分裂指数衡量）的丧失；然而，γ干扰素的产生部分恢复。与先前在太空中进行的一项实验相反，IL-1的产生没有受到抑制。
