Kawazu K, Zhang H, Kanzaki H
Laboratory of Bioresources Chemistry, Faculty of Agriculture, Okayama University, Japan.
Biosci Biotechnol Biochem. 1996 Sep;60(9):1410-2. doi: 10.1271/bbb.60.1410.
The generation and accumulation of both benzoic acid (BA) and its conjugates were induced in suspension cultured cells of Pinus thunbergii by administering either phenylacetic acid (PA), a toxic metabolite of Bacillus cereus (strain HY-3) accompanying the pine wood nematode, or a lyophilized culture supernatant of this bacterium. BA conjugates reached their maximal levels in quantity two days after the administration and then decreased gradually until the 14th day, while BA increased significantly throughout this period. This pattern is similar to that in 3-year-old pine trees treated with PA, suggesting that the pathological reaction of pine tissues to the PA toxin might be involved in the pathogenesis mechanism for the pine wilt disease.
通过施用苯乙酸（PA）（一种伴随松材线虫的蜡样芽孢杆菌（菌株HY - 3）的有毒代谢产物）或该细菌的冻干培养上清液，诱导黑松悬浮培养细胞中苯甲酸（BA）及其共轭物的生成和积累。施用后两天，BA共轭物的量达到最高水平，然后逐渐下降直至第14天，而在此期间BA显著增加。这种模式与用PA处理的3年生松树中的模式相似，这表明松树组织对PA毒素的病理反应可能参与了松材线虫病的发病机制。
