Kawazu K, Zhang H, Yamashita H, Kanzaki H
Laboratory of Bioresources Chemistry, Faculty of Agriculture, Okayama University, Japan.
Biosci Biotechnol Biochem. 1996 Sep;60(9):1413-5. doi: 10.1271/bbb.60.1413.
Phenylacetic acid (PA), a toxin produced by three strains of bacteria accompanying the pine wood nematode, Bursaphelenchus xylophilus, was found to be formed in a culture of the nematode. An animal nutrient, nutrient broth (NB) medium, was more suitable for PA production of the accompanying bacteria than a vegetable nutrient, potato sucrose malt extract (PSM) medium. It is presumed that dead nematodes in the PSM medium provided the bacteria with nutrient for PA production. In the culture of virulent isolate OKD-3, more PA was detected than in that of less-virulent isolate OKD-1. PA production of the accompanying bacteria can dominate the pathogenicity of the nematode.
苯乙酸（PA）是松材线虫（Bursaphelenchus xylophilus）携带的三种细菌产生的毒素，已发现其在线虫培养物中形成。动物营养物肉汤培养基（NB）比植物营养物马铃薯蔗糖麦芽提取物培养基（PSM）更适合携带细菌产生PA。据推测，PSM培养基中的死线虫为细菌提供了产生PA的营养物质。在强毒分离株OKD-3的培养物中，检测到的PA比弱毒分离株OKD-1的培养物中更多。携带细菌产生PA的能力可以主导线虫的致病性。
