O'Donnell-Maloney M J, Smith C L, Cantor C R
Sequenom, Inc., Boston, MA 02110, USA.
Trends Biotechnol. 1996 Oct;14(10):401-7. doi: 10.1016/0167-7799(96)10052-4.
Microfabricated arrays of immobilized oligodeoxynucleotide probes are proving to be a powerful tool for rapidly generating sequence data via hybridization. These arrays are made either by immobilization of the probe post-synthetically, or by in situ synthesis of the probe. Hybridization of the target is easily achieved on the arrays, with analysis proceeding either by direct detection, or through enzyme-mediated detection; analysis of the hybridization pattern yields sequence information about the target. Such facile and rapid data acquisition will assist the challenging task of sequencing the human genome, and also will lead to a new generation of diagnostic assays.
固定化寡脱氧核苷酸探针的微制造阵列正被证明是一种通过杂交快速生成序列数据的强大工具。这些阵列可以通过合成后固定探针来制备，也可以通过探针的原位合成来制备。靶标的杂交在阵列上很容易实现，分析可以通过直接检测或酶介导检测进行；杂交模式的分析产生关于靶标的序列信息。如此简便快速的数据采集将有助于完成人类基因组测序这一具有挑战性的任务，还将催生新一代诊断检测方法。
