Erbe J L, Taylor K B, Hall L M
Department of Biochemistry and Molecular Genetics, University of Alabama at Birmingham 35294, USA.
J Ind Microbiol. 1996 Jul;17(1):41-6. doi: 10.1007/BF01570147.
A cDNA encoding mouse metallothionein was cloned into the shuttle vector pUc303, creating a translational fusion with the bacterial chloramphenicol acetyltransferase gene. The resulting fusion protein has been expressed in the cyanobacterium Synechococcus PCC7942. Cyanobacterial transformants expressed mouse metallothionein-specific mRNA species as detected by RNA slot blots. In addition, the transformants expressed a unique cadmium ion-binding protein corresponding to the predicted size of the mouse metallothionein fusion protein. Expression of this fusion protein conferred a two- to five-fold increase in cadmium ion tolerance and accumulation on Synechococcus PCC7942.
将编码小鼠金属硫蛋白的cDNA克隆到穿梭载体pUc303中，与细菌氯霉素乙酰转移酶基因形成翻译融合体。所得融合蛋白已在蓝藻聚球藻PCC7942中表达。通过RNA斑点杂交检测到蓝藻转化体表达小鼠金属硫蛋白特异性mRNA种类。此外，转化体表达一种独特的镉离子结合蛋白，其大小与预测的小鼠金属硫蛋白融合蛋白大小相符。这种融合蛋白的表达使聚球藻PCC7942对镉离子的耐受性和积累增加了两到五倍。
