产脂固氮螺菌的转运RNA：转运RNA种类的分级分离与鉴定以及天冬酰胺转运RNA（QUU）的核苷酸序列

Azospirillum lipoferum tRNAs: fractionation and identification of tRNA species and the nucleotide sequence of tRNA(Asn) (QUU).

作者信息

Sivakamasundari R, Jayabaskaran C

机构信息

Department of Biochemistry, Indian Institute of Science, Bangalore, India.

出版信息

Biochem Mol Biol Int. 1996 Dec;40(6):1221-6. doi: 10.1080/15216549600201863.

DOI:10.1080/15216549600201863

PMID:8988334

Abstract

Transfer RNAs of Azospirillum lipoferum were separated by two-dimensional gel electrophoresis and identified by aminoacylation. Thirty-six tRNA spots were resolved by this technique and twenty-six tRNA species have been identified. There are five tRNAs for Leu, four for Val, three for Pro, two each for Arg, Ile, Lys and Tyr, and one each for Ala, Asp, His, Phe, Ser and Thr. The tRNA (Asn) (QUU) was purified and its nucleotide sequence was determined. The A. lipoferum tRNA (Asn) (QUU) is 92% similar to B. subtilis tRNA (Asn) gene and two hypermodified nucleosides, queuosine (Q) and N-(9-beta-D Ribofuranosylpurine-6-YL)-carbamoyl)-threonine (t6A) are present in this tRNA.

摘要

通过二维凝胶电泳分离了脂环固氮螺菌的转运RNA，并通过氨酰化进行鉴定。用该技术分辨出36个tRNA斑点，已鉴定出26种tRNA。有5种tRNA对应亮氨酸，4种对应缬氨酸，3种对应脯氨酸，精氨酸、异亮氨酸、赖氨酸和酪氨酸各有2种，丙氨酸、天冬氨酸、组氨酸、苯丙氨酸、丝氨酸和苏氨酸各有1种。纯化了tRNA（Asn）（QUU）并测定了其核苷酸序列。脂环固氮螺菌的tRNA（Asn）（QUU）与枯草芽孢杆菌tRNA（Asn）基因有92%的相似性，且该tRNA中存在两种超修饰核苷，即queuosine（Q）和N-（9-β-D-呋喃核糖基嘌呤-6-YL）-氨基甲酰基）-苏氨酸（t6A）。