Kent S P, McKibbin J M, Boggio R, McKibbin J B
Vox Sang. 1977 Oct;33(4):193-201. doi: 10.1111/j.1423-0410.1977.tb04463.x.
Erythrocytic membranes from blood group A individuals were assayed for A antigen using a quantitative hemagglutination inhibition technique. The membranes were then extracted for lipid and glycoprotein. Although some A antigen was usually found in the glycoprotein fraction, most of the activity was in the lipid fraction. The sum of A antigen activity in the lipid, glycoprotein, and membrane residue fractions only occasionally was equal to the A activity in the erythrocytic ghosts. However, when certain lipid preparations with little or no A antigen (enhancement factors) were added to the glycolipid fractions, the amount of A antigen demonstrated was usually greatly increased. Under these conditions, the sum of the fractions often was much greater than the A antigen demonstrated in erythrocytic membranes. This suggests that the organization or arrangement of A antigenic determinants in the red cell membrane may not always permit a stoichiometric reaction with anti-A molecules.
使用定量血凝抑制技术对 A 血型个体的红细胞膜进行 A 抗原检测。然后从这些膜中提取脂质和糖蛋白。虽然在糖蛋白部分通常能发现一些 A 抗原，但大部分活性存在于脂质部分。脂质、糖蛋白和膜残余部分中 A 抗原活性的总和仅偶尔与红细胞空壳中的 A 活性相等。然而，当将某些几乎没有或没有 A 抗原的脂质制剂（增强因子）添加到糖脂部分时，所显示的 A 抗原量通常会大幅增加。在这些条件下，各部分的总和往往远大于红细胞膜中所显示的 A 抗原。这表明红细胞膜中 A 抗原决定簇的组织或排列可能并不总是允许与抗 A 分子进行化学计量反应。
