Ohtani S, Ohhira H, Watanabe A, Ogasawara A, Sugimoto H
Department of Forensic Medicine, Kanagawa Dental College, Japan.
J Forensic Sci. 1997 Jan;42(1):137-9.
To determine the age of a subject from teeth accurately utilizing the racemization rates of amino acids, standard samples of the same tooth species from the same jaw are necessary as controls, as well as data for identification. However, standard teeth are generally stored in fixatives such as ethanol and formalin. We investigated and compared the degree of progression of racemization of dentinal aspartic acid in teeth stored in 95% ethanol, 10% formalin, or 10% neutral formalin fixatives. The racemization rate of dentinal aspartic acid in teeth stored in 10% neutral formalin was the highest, followed by that for teeth stored in 10% formalin then that for teeth stored in 95% ethanol. Teeth stored in these fixatives at 15 degrees C showed almost no progression of racemization. The racemization ratio (D/L ratio) in teeth extracted 10 years previously was almost unchanged from that at the time of extraction, and allowed an accurate evaluation of the subjects age at tooth extraction.
为了利用氨基酸的消旋化速率准确地从牙齿确定受试者的年龄，需要来自同一颌骨的相同牙齿种类的标准样本作为对照以及用于识别的数据。然而，标准牙齿通常保存在乙醇和福尔马林等固定剂中。我们研究并比较了保存在95%乙醇、10%福尔马林或10%中性福尔马林固定剂中的牙齿中牙本质天冬氨酸的消旋化进展程度。保存在10%中性福尔马林中的牙齿中牙本质天冬氨酸的消旋化速率最高，其次是保存在10%福尔马林中的牙齿，然后是保存在95%乙醇中的牙齿。在15摄氏度下保存在这些固定剂中的牙齿几乎没有消旋化进展。10年前拔除的牙齿中的消旋化比率（D/L比率）与拔牙时几乎没有变化，并且能够准确评估受试者拔牙时的年龄。
