从瑞士乳杆菌CP53中分离并鉴定一种质粒。

Isolation and characterization of a plasmid from Lactobacillus helveticus CP53.

作者信息

Yamamoto N, Takano T

机构信息

R & D Center, Calpis Food Industry Co., Ltd., Kanagawa, Japan.

出版信息

Biosci Biotechnol Biochem. 1996 Dec;60(12):2069-70. doi: 10.1271/bbb.60.2069.

DOI:10.1271/bbb.60.2069

PMID:8988641

Abstract

A plasmid, pCP53 (11.5 kilo base pairs long), was isolated from Lactobacillus helveticus CP53 and its restriction map was constructed. A chimeric plasmid, pCP53D (4.7 kb), which had a 2.0-kb HindIII fragment from pCP53 and a tetracycline resistance (Tc) gene from Streptococcus faecalis replicated and expressed tetracycline resistance in Lactobacillus casei CP680. Fifteen strains of L. helveticus were transformed with pCP53D by electroporation and selected for Tc resistance. Transformants were obtained from two out of 15 host strains, L. helveticus CP611 and CP791, with 5 x 10(3) and 50 transformants per microgram of DNA, respectively.

摘要

从瑞士乳杆菌CP53中分离出一个质粒pCP53（长11.5千碱基对），并构建了其限制酶切图谱。一个嵌合质粒pCP53D（4.7 kb），它含有来自pCP53的2.0 kb HindIII片段和来自粪肠球菌的四环素抗性（Tc）基因，该质粒在干酪乳杆菌CP680中复制并表达四环素抗性。通过电穿孔法用pCP53D转化了15株瑞士乳杆菌，并筛选出对Tc有抗性的菌株。从15个宿主菌株中的两个，即瑞士乳杆菌CP611和CP791获得了转化子，每微克DNA分别有5×10³和50个转化子。