Fenn J P, Segal H, Blevins L, Fawson S, Newcomb-Gayman P, Carroll K C
Department of Pathology, University of Utah School of Medicine, Salt Lake City 84132, USA.
Diagn Microbiol Infect Dis. 1996 Jan;24(1):31-5. doi: 10.1016/0732-8893(95)00199-9.
A total of 502 yeast isolates were tested with the 30-min MUREX Candida albicans CA50 (Norcross, GA) test for presumptive identification of C. albicans. The results were compared with the standard 2-h germ tube test, which was the reference standard. Of the 502 isolates, 316 were C. albicans and 186 were non-C. albicans. Identifications were based on germ tube reactions; the API20C and chlamydospore agars were used when discrepant results persisted between the germ tube and MUREX test after repeat testing of the MUREX method. A total of 16 C. albicans gave negative results on initial testing with the MUREX test but were interpreted as positive when repeated. Three germ tube negative yeasts initially tested positive with the MUREX but were negative when repeated. Two additional yeast isolates gave incorrect results with the MUREX, even with repeat testing: C. albicans and C. lusitaniae. The initial sensitivity and specificity for the MUREX C. albicans CA50 test were 94.6% and 97.8%, respectively. As an addition to the study, two fetal bovine sera were compared for production of germ tubes; fetal bovine serum and Fetal Clone II. The testing found them to be in 100% agreement.
共有502株酵母分离株接受了30分钟的MUREX白色念珠菌CA50（美国佐治亚州诺克罗斯）试验，以进行白色念珠菌的初步鉴定。将结果与标准的2小时芽管试验进行比较，后者为参考标准。在这502株分离株中，316株为白色念珠菌，186株为非白色念珠菌。鉴定基于芽管反应；当MUREX方法重复测试后芽管试验和MUREX试验结果仍存在差异时，使用API20C和厚壁孢子琼脂。共有16株白色念珠菌在初次MUREX试验中结果为阴性，但重复试验时被判定为阳性。3株芽管阴性的酵母最初MUREX试验呈阳性，但重复试验时为阴性。另外2株酵母分离株即使经过重复测试，MUREX试验结果仍不正确：白色念珠菌和葡萄牙念珠菌。MUREX白色念珠菌CA50试验的初始敏感性和特异性分别为94.6%和97.8%。作为该研究的补充，比较了两种胎牛血清诱导芽管产生的情况；胎牛血清和Fetal Clone II。测试发现它们的结果完全一致。
