Zellweger R, Wichmann M W, Ayala A, Stein S, DeMaso C M, Chaudry I H
Department of Surgery, Michigan State University, East Lansing, USA.
Crit Care Med. 1997 Jan;25(1):106-10. doi: 10.1097/00003246-199701000-00021.
To determine: a) whether the cell-mediated immune response during sepsis differs in females vs. males; and b) whether the survival rate in females is different than in males after a septic insult.
A prospective, randomized animal study.
University research laboratory.
Male and female proestrus C3H/HeN mice.
After anesthesia, male and proestrus female mice underwent cecal ligation puncture to induce sepsis. The mice were killed at 24 hrs after the onset of sepsis.
Splenocyte proliferation, as well as splenocyte interleukin (IL)-2 and IL-3 release, was determined by bioassay. In additional studies, survival rate after septic challenge was measured over 10 days. Splenocyte proliferative capacity and splenocyte IL-2 and IL-3 release were markedly decreased in male, but not in female, septic mice. Furthermore, the survival rate of septic female proestrus mice was significantly higher than in comparable male mice.
These results support the concept that the immune response of females differs from males, and that females are immunologically better positioned to meet the challenge of sepsis.
确定：a）脓毒症期间细胞介导的免疫反应在雌性与雄性中是否存在差异；b）脓毒症发作后雌性的存活率与雄性是否不同。
一项前瞻性随机动物研究。
大学研究实验室。
处于动情前期的雄性和雌性C3H/HeN小鼠。
麻醉后，对雄性和处于动情前期的雌性小鼠进行盲肠结扎穿刺以诱导脓毒症。脓毒症发作24小时后处死小鼠。
通过生物测定法测定脾细胞增殖以及脾细胞白细胞介素（IL）-2和IL-3的释放。在另外的研究中，在10天内测量脓毒症攻击后的存活率。雄性脓毒症小鼠的脾细胞增殖能力以及脾细胞IL-2和IL-3的释放明显降低，而雌性则不然。此外，脓毒症雌性动情前期小鼠的存活率显著高于相应的雄性小鼠。
这些结果支持以下观点，即雌性的免疫反应与雄性不同，并且雌性在免疫方面更有能力应对脓毒症的挑战。
