酵母多态性STA基因在减数分裂重组体中的分离及糖化酶产生的分析。

Segregation of yeast polymorphic STA genes in meiotic recombinants and analysis of glucoamylase production.

作者信息

Balogh I, Maráz A

机构信息

Department of Microbiology and Biotechnology, University of Horticulture and Food Industry, Budapest, Hungary.

出版信息

Can J Microbiol. 1996 Dec;42(12):1190-6. doi: 10.1139/m96-153.

DOI:10.1139/m96-153

PMID:8989859

Abstract

Hybrid yeast strains were constructed using haploid Saccharomyces cerevisiae and Saccharomyces cerevisiae var. diastaticus strains to get haploid meiotic recombinants having more than one copy of STA1, STA2, and STA3 genes. STA genes were localized on the chromosomes by pulsed field gel electrophoresis. Working gene dosage effects were found among STA genes in liquid starch medium, indicating low levels of glucose repression. Growth of strains, however, was not influenced by their STA copy number.

摘要

使用单倍体酿酒酵母和糖化酵母菌株构建杂种酵母菌株，以获得具有多个STA1、STA2和STA3基因拷贝的单倍体减数分裂重组体。通过脉冲场凝胶电泳将STA基因定位在染色体上。在液体淀粉培养基中发现STA基因之间存在有效基因剂量效应，表明葡萄糖阻遏水平较低。然而，菌株的生长不受其STA拷贝数的影响。