从寡核苷酸形状到基因组SELEX：新型生物调节环
From oligonucleotide shapes to genomic SELEX: novel biological regulatory loops.
作者信息
Gold L, Brown D, He Y, Shtatland T, Singer B S, Wu Y
机构信息
University of Colorado at Boulder, Department of Molecular, Cellular, and Developmental Biology, 80309-0347, USA.
出版信息
Proc Natl Acad Sci U S A. 1997 Jan 7;94(1):59-64. doi: 10.1073/pnas.94.1.59.
Abstract
The SELEX method and oligonucleotide combinatorial chemistry discovery process yields high-affinity/high-specificity ligands for virtually any molecular target. Typically, the enormous starting libraries used in the SELEX process contain 10(14)-10(15) sequences. We now ask if the smaller sequences, complexity of extant organisms, and evolutionary history provide useful interactions between oligonucleotides and at least some unexpected targets. That is, do organisms contain a robust "linkage map" between their oligonucleotides and proteins and/or small molecules that enriches life?
摘要
SELEX方法和寡核苷酸组合化学发现过程可为几乎任何分子靶标产生高亲和力/高特异性配体。通常，SELEX过程中使用的庞大起始文库包含10（14）-10（15）个序列。我们现在要问，较小的序列、现存生物的复杂性以及进化历史是否能在寡核苷酸与至少一些意想不到的靶标之间提供有用的相互作用。也就是说，生物体内的寡核苷酸与蛋白质和/或小分子之间是否存在丰富生命的强大“连接图谱”？
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