一种与酵母Cdc6p相关的人类蛋白质。
A human protein related to yeast Cdc6p.
作者信息
Williams R S, Shohet R V, Stillman B
机构信息
Cold Spring Harbor Laboratory, Cold Spring Harbor, NY 11724, USA.
出版信息
Proc Natl Acad Sci U S A. 1997 Jan 7;94(1):142-7. doi: 10.1073/pnas.94.1.142.
Abstract
The unstable proteins Cdc6p and cdc18+ are essential and rate limiting for the initiation of DNA replication in Saccharomyces cerevisiae and Schizosaccharomyces pombe, respectively, and also participate in checkpoint controls that ensure DNA replication is completed before mitosis is initiated. We have identified Xenopus and human proteins closely related to Cdc6p/cdc18. The human protein, p62(cdc6), is encoded on chromosome 17q21.3 and includes putative cyclin-dependent kinase phosphorylation sites, destruction boxes, a nucleotide binding/ATPase domain, and a potential leucine zipper. Expression of p62(cdc6) mRNA and protein is suppressed in human diploid fibroblasts made quiescent by serum starvation, and peaks as cells reenter the cell cycle and replicate DNA following serum stimulation. Conservation of structure among proteins involved in initiation suggests that fundamental features of replication complexes are maintained in all eukaryotes.
摘要
不稳定蛋白Cdc6p和cdc18 +分别对于酿酒酵母和粟酒裂殖酵母中DNA复制的起始至关重要且具有限速作用，并且还参与确保在有丝分裂开始之前完成DNA复制的检查点控制。我们已经鉴定出与Cdc6p / cdc18密切相关的非洲爪蟾和人类蛋白。人类蛋白p62（cdc6）在17q21.3染色体上编码，包括假定的细胞周期蛋白依赖性激酶磷酸化位点、破坏盒、核苷酸结合/ATP酶结构域和潜在的亮氨酸拉链。在因血清饥饿而静止的人类二倍体成纤维细胞中，p62（cdc6）mRNA和蛋白的表达受到抑制，并且在血清刺激后细胞重新进入细胞周期并复制DNA时达到峰值。参与起始的蛋白质之间结构的保守性表明，复制复合物的基本特征在所有真核生物中都得以保留。
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