Goldsmith P, Bunker C, Leslie T, Foreman J, Dowd P M
Department of Dermatology, University College London Medical School, UK.
Skin Pharmacol. 1996;9(5):289-97. doi: 10.1159/000211427.
The effect of topical application of clobetasol propionate ointment (0.05% w/v) on the vascular changes induced by intradermal injections of histamine, calcitonin gene-related peptide, substance P, endothelin-1 and compound 48/80 was studied. Clobetasol propionate ointment was applied topically under occlusion to the forearm skin of healthy volunteers and vehicle base was applied to the contralateral forearm. The intradermal injections were made 4 h or, in a separate study, 72 h after topical steroid application. Responses were measured by planimetry and laser Doppler flowmetry. Four hours application of steroid did not significantly alter the responses to any of the vasoactive substances. After 72 hours application, clobetasol propionate significantly increased the size of the endothelin-1-induced area of vasoconstriction (p < 0.02) and significantly reduced the size of the flares induced by endothelin-1 (p < 0.02), substance P (p < 0.009) and compound 48/80 (p < 0.05). We conclude that the most likely explanation of our data is an inhibition by the steroid of cutaneous mast cell function.
研究了局部应用丙酸氯倍他索软膏（0.05% w/v）对皮内注射组胺、降钙素基因相关肽、P物质、内皮素-1和化合物48/80所诱导的血管变化的影响。将丙酸氯倍他索软膏在封闭条件下局部应用于健康志愿者的前臂皮肤，而将赋形剂基质应用于对侧前臂。在局部应用类固醇4小时后，或在另一项研究中在72小时后进行皮内注射。通过平面测量法和激光多普勒血流仪测量反应。应用类固醇4小时并未显著改变对任何血管活性物质的反应。应用72小时后，丙酸氯倍他索显著增加了内皮素-1诱导的血管收缩面积大小（p < 0.02），并显著减小了内皮素-1（p < 0.02）、P物质（p < 0.009）和化合物48/80（p < 0.05）所诱导的风团大小。我们得出结论，对我们数据最可能的解释是类固醇对皮肤肥大细胞功能的抑制作用。
