采用杀螺旋体抗体试验、流式细胞术间接荧光抗体测定法和免疫印迹法检测抗伯氏疏螺旋体抗体反应。
Detection of anti-Borrelia burgdorferi antibody responses with the borreliacidal antibody test, indirect fluorescent-antibody assay performed by flow cytometry, and western immunoblotting.
作者信息
Creson J R, Lim L C, Glowacki N J, Callister S M, Schell R F
机构信息
Wisconsin State Laboratory of Hygiene, University of Wisconsin, Madison 53706, USA.
出版信息
Clin Diagn Lab Immunol. 1996 Mar;3(2):184-90. doi: 10.1128/cdli.3.2.184-190.1996.
Abstract
Borreliacidal antibodies participate in the resolution of Lyme disease by clearing Borrelia burgdorferi sensu lato from the host. Detection of borreliacidal antibodies is also valuable for determination of the specific serodiagnosis of Lyme disease. We show in this work that antibody detected by the borreliacidal antibody test did not correlate with antibody detected by the indirect fluorescent-antibody assay or Western immunoblotting. Detection of borreliacidal antibody decreased with elimination of the spirochete from the host in the presence or absence of therapy. By contrast, the antibody responses detected by the indirect fluorescent-antibody assay or Western immunoblotting remained elevated or continued to expand, respectively. This suggests that the borreliacidal antibody test is a prognostic indicator for clearance of the spirochete. Additional investigations with humans are needed to confirm the prognostic potential of the borreliacidal antibody test.
摘要
杀疏螺旋体抗体通过清除宿主体内的伯氏疏螺旋体狭义种参与莱姆病的消退。检测杀疏螺旋体抗体对于莱姆病的特异性血清学诊断也很有价值。我们在这项研究中表明，杀疏螺旋体抗体试验检测到的抗体与间接荧光抗体试验或Western免疫印迹检测到的抗体不相关。在有或没有治疗的情况下，随着宿主体内螺旋体的清除，杀疏螺旋体抗体的检测减少。相比之下，间接荧光抗体试验或Western免疫印迹检测到的抗体反应分别保持升高或继续扩大。这表明杀疏螺旋体抗体试验是螺旋体清除的一个预后指标。需要对人类进行更多研究以证实杀疏螺旋体抗体试验的预后潜力。
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Detection of anti-Borrelia burgdorferi antibody responses with the borreliacidal antibody test, indirect fluorescent-antibody assay performed by flow cytometry, and western immunoblotting.采用杀螺旋体抗体试验、流式细胞术间接荧光抗体测定法和免疫印迹法检测抗伯氏疏螺旋体抗体反应。
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本文引用的文献
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In vitro inhibition of Borrelia burgdorferi growth by antibodies.抗体对伯氏疏螺旋体生长的体外抑制作用。
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Characterization of the borreliacidal antibody response to Borrelia burgdorferi in humans: a serodiagnostic test.人类对伯氏疏螺旋体的杀螺旋体抗体反应的特征：一项血清诊断试验。
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Role of attached lipid in immunogenicity of Borrelia burgdorferi OspA.附着脂质在伯氏疏螺旋体OspA免疫原性中的作用。
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The 39-kilodalton protein of Borrelia burgdorferi: a target for bactericidal human monoclonal antibodies.伯氏疏螺旋体的39千道尔顿蛋白：杀菌性人单克隆抗体的一个靶点。
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Western blotting in the serodiagnosis of Lyme disease.蛋白质免疫印迹法在莱姆病血清学诊断中的应用
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FEMS Immunol Med Microbiol. 1993 Jun;7(1):67-71. doi: 10.1111/j.1574-695X.1993.tb00383.x.
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Vaccine. 1993;11(10):1049-54. doi: 10.1016/0264-410x(93)90132-h.
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JAMA. 1994 Jun 8;271(22):1764-8.
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