利用流式细胞术检测仓鼠体内的杀博氏疏螺旋体抗体。
Detection of borreliacidal antibodies in hamsters by using flow cytometry.
作者信息
Sachsenmeier K F, Schell K, Morrissey L W, Pennell D R, West R M, Callister S M, Schell R F
机构信息
Wisconsin State Laboratory of Hygiene, Department of Medical Microbiology and Immunology, University of Wisconsin, Madison 53706.
出版信息
J Clin Microbiol. 1992 Jun;30(6):1457-61. doi: 10.1128/jcm.30.6.1457-1461.1992.
Abstract
Flow cytometry can be used to detect antibody that kills Borrelia burgdorferi. Borreliacidal activity was detected within 3 h of incubating B. burgdorferi with immune serum and complement. Right-angle light scatter and propidium iodide fluorescence were the cytometric parameters which correlated best with in vitro killing of B. burgdorferi. Flow cytometry is a rapid method for determining the presence of borreliacidal activity and may lead to a better serodiagnostic test for the detection of Lyme disease.
摘要
流式细胞术可用于检测杀灭伯氏疏螺旋体的抗体。在将伯氏疏螺旋体与免疫血清及补体孵育3小时内即可检测到杀螺旋体活性。直角光散射和碘化丙啶荧光是与体外杀灭伯氏疏螺旋体最相关的细胞计数参数。流式细胞术是一种确定杀螺旋体活性存在的快速方法，可能会带来更好的用于检测莱姆病的血清学诊断试验。
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本文引用的文献
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The spirochetal etiology of Lyme disease.莱姆病的螺旋体病因
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