肺炎球菌表面黏附素A（PsaA）的纯化及血清反应性
Purification and seroreactivity of pneumococcal surface adhesin A (PsaA).
作者信息
Tharpe J A, Russell H
机构信息
Childhood and Respiratory Diseases Branch, Centers for Disease Control and Prevention, Atlanta, Georgia 30333, USA.
出版信息
Clin Diagn Lab Immunol. 1996 Mar;3(2):227-9. doi: 10.1128/cdli.3.2.227-229.1996.
Abstract
Pneumococcal surface adhesin A (PsaA) is a 37-kDa common protein antigen of Streptococcus pneumoniae. In the present study, the protein was purified so that its immunoreactivity could be determined. PsaA was released and purified from cells by lysis in the presence of n-laurylsarcosine; this was followed by ammonium sulfate precipitation and subsequent preparative isoelectric focusing. A capture antibody enzyme-linked immunosorbent assay was used to determine the immunoreactivity of purified PsaA. The assay had a 67% sensitivity for sera from patients with bacteremic pneumococcal pneumonia. A specificity of 97% was estimated on the basis of a lack of reactivity with sera from patients with pneumonia caused by other organisms. PsaA is a potential vaccine candidate and may be useful as an antigen in a diagnostic assay for pneumococcal disease.
摘要
肺炎球菌表面黏附素A（PsaA）是肺炎链球菌的一种37 kDa常见蛋白抗原。在本研究中，对该蛋白进行了纯化，以便确定其免疫反应性。通过在月桂酰肌氨酸存在下裂解细胞来释放并纯化PsaA；随后进行硫酸铵沉淀及后续的制备性等电聚焦。采用捕获抗体酶联免疫吸附测定法来确定纯化后的PsaA的免疫反应性。该测定法对菌血症性肺炎球菌肺炎患者血清的敏感性为67%。基于与其他病原体所致肺炎患者血清无反应性，估计其特异性为97%。PsaA是一种潜在的疫苗候选物，可能作为肺炎球菌疾病诊断测定中的一种抗原发挥作用。
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