对核黄素缺乏大鼠小肠中所见形态学和细胞动力学变化的可逆性研究。
An investigation into the reversibility of the morphological and cytokinetic changes seen in the small intestine of riboflavin deficient rats.
作者信息
Williams E A, Rumsey R D, Powers H J
机构信息
University Department of Paediatrics, Sheffield Children's Hospital.
出版信息
Gut. 1996 Aug;39(2):220-5. doi: 10.1136/gut.39.2.220.
BACKGROUND
Impaired iron handling in riboflavin deficiency is thought to be partially a result of significant morphological and cytokinetic changes within the small intestine.
AIMS
The aim of the study was to find out if the responses of the rat small intestine to riboflavin deficiency induced at weaning could be reversed upon repletion.
SUBJECTS
48 female weanling Wistar rats were used for the purpose of the study.
METHODS
Rats were fed a riboflavin deficient diet or a complete control diet for a period of five weeks followed by a repletion period of up to three weeks. Rats were killed on day 0, 2, 7, or 21 of repletion. The duodenum was removed and fixed for subsequent analysis.
RESULTS
Five weeks of riboflavin deficiency significantly changed the morphology and cytokinetics of the duodenum; the changes were not reversed within the 21 day repletion period despite biochemical evidence for a correction of the deficiency.
CONCLUSIONS
The results show that the small intestine cannot readily recover from a period of riboflavin deficiency induced at weaning, supporting the notion that the weaning period is a critical time for gastrointestinal development and highlighting the importance of adequate nutrition during infancy.
背景
核黄素缺乏时铁代谢受损被认为部分是小肠内显著的形态学和细胞动力学变化所致。
目的
本研究旨在查明断奶时诱导的大鼠小肠对核黄素缺乏的反应在补充核黄素后是否可以逆转。
对象
48只雌性断乳Wistar大鼠用于本研究。
方法
大鼠分别喂食核黄素缺乏饮食或完全对照饮食5周，随后进行长达3周的补充期。在补充期的第0、2、7或21天处死大鼠。取出十二指肠并固定以备后续分析。
结果
5周的核黄素缺乏显著改变了十二指肠的形态和细胞动力学；尽管有生化证据表明缺乏得到纠正，但在21天的补充期内这些变化并未逆转。
结论
结果表明，小肠不能轻易从断奶时诱导的核黄素缺乏期恢复，支持断奶期是胃肠道发育关键时期的观点，并突出了婴儿期充足营养的重要性。
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