Tamura A
Department of Radiology, Saitama Medical School.
Nihon Igaku Hoshasen Gakkai Zasshi. 1996 Mar;56(4):183-6.
After evaluating ex vivo pig liver by 1H magnetic resonance spectroscopy (1H-MRS) using a 1.5 Tesla super-conducting unit, the assignment of peaks was reevaluated in vitro using a 9.4 Tesla superconducting unit. The portal vein was cannulated and perfused by cooled Euro-Collins solution, and pig liver was removed and preserved in the solution. Five to 8 g of the liver was excised before and after preservation, then extracted by perchloric acid (PCA). In 1H-MRS of PCA extracted pig liver, large peaks of fatty acid disappeared, while peaks of the choline group from 3.2-3.3 ppm were clearly observed. Using high performance liquid chromatography (HPLC) and 31P (phosphorus)-MRS, the 3.23 ppm peak was determined as glycerophosphorylcholine (GPC), which diminished after preservation. The chronological change of GPC in PCA extracted pig liver was able to be observed by 1H-MRS. GPC peak may play a role as an indicator in evaluating preserved liver by 1H-MRS.
使用1.5特斯拉超导装置通过1H磁共振波谱（1H-MRS）对离体猪肝进行评估后，使用9.4特斯拉超导装置在体外重新评估峰的归属。将门静脉插管并用冷却的欧洲柯林斯溶液灌注，然后取出猪肝并保存在该溶液中。在保存前后切除5至8克肝脏，然后用高氯酸（PCA）提取。在PCA提取的猪肝的1H-MRS中，脂肪酸的大峰消失，而在3.2-3.3 ppm处胆碱基团的峰清晰可见。使用高效液相色谱（HPLC）和31P（磷）-MRS，将3.23 ppm的峰确定为甘油磷酸胆碱（GPC），其在保存后减少。通过1H-MRS可以观察到PCA提取的猪肝中GPC的时间变化。GPC峰可能作为通过1H-MRS评估保存肝脏的指标发挥作用。
