Yokota Hajime, Guo Jianfei, Matoba Munetaka, Higashi Kotaro, Tonami Hisao, Nagao Yosinobu
Department of Radiology, Kanazawa Medical University, Ishikawa 920-0293, Japan.
Radiat Med. 2005 Dec;23(8):590-3.
This study was performed to investigate the lactate, creatine, and choline changes in excised rabbit lung metabolites using proton magnetic resonance spectroscopy (1H-MRS).
Rabbit lung was preserved with -20 degrees C 10% dimethyl sulfoxide (DMSO) solution until used, and the lactate, creatine, and choline levels of the rabbit lung were evaluated by 1H-MRS (6.34T superconducting unit). The control of lung tissue was excised under low-temperature conditions of liquid nitrogen, and preserved in 10% DMSO solution at -20 degrees C until evaluated. The lung tissues were excised at room temperature, and 30 min later, the tissues were saved in 10% DMSO solution at -20 degrees C until evaluated, and the lactate, creatine, and choline were measured by 1H-MRS after 20, 40, 60 and 80 days.
The lactate, creatine, and choline in the control tissue measured 0.412 micromol/g, 0.024 micromol/g and 0.696 micromol/g, respectively. The lactate, creatine, and choline in the experimental tissue measured 0.414 micromol/g, 0.029 micromol/g and 0.388 micromol/g after 60 days, respectively.
In this study, the level of lactate within tissues preserved in 10% DMSO solution at -20 degrees C achieved a state of equilibrium within 60 days.
本研究旨在利用质子磁共振波谱（1H-MRS）研究切除的兔肺代谢物中乳酸、肌酸和胆碱的变化。
兔肺用-20℃的10%二甲亚砜（DMSO）溶液保存至使用，通过1H-MRS（6.34T超导单元）评估兔肺的乳酸、肌酸和胆碱水平。在液氮低温条件下切除肺组织对照，并保存在-20℃的10% DMSO溶液中直至评估。在室温下切除肺组织，30分钟后，将组织保存在-20℃的10% DMSO溶液中直至评估，并在20、40、60和80天后通过1H-MRS测量乳酸、肌酸和胆碱。
对照组织中的乳酸、肌酸和胆碱分别为0.412微摩尔/克、0.024微摩尔/克和0.696微摩尔/克。60天后，实验组织中的乳酸、肌酸和胆碱分别为0.414微摩尔/克、0.029微摩尔/克和0.388微摩尔/克。
在本研究中，在-20℃的10% DMSO溶液中保存的组织内乳酸水平在60天内达到平衡状态。
