Calingasan N Y, Bernstein J J, Blass J P
Will Rogers Institute, Cornell University Medical College, Burke Medical Research Institute, White Plains, NY, USA.
J Neurol Sci. 1996 Dec;144(1-2):21-3. doi: 10.1016/s0022-510x(96)00189-x.
Human leptomeningeal (arachnoid and pia mater) cells in culture have been demonstrated in replicated studies to express typical neuronal proteins such as neurofilament protein and neuron-specific enolase. In addition, they can express glial fibrillary acidic protein. The present study examines the possibility that neuronal and glial proteins might be present in rat and human leptomeningeal cells in situ. The neuronal proteins 160 kDa and 200 kDa neurofilaments, neuron-specific enolase and microtubule-associated protein 2 were, however, not immunolocalized in either the pia mater or arachnoid. Glial fibrillary acidic protein and galactocerebroside were also not detected, while fibronectin and vimentin immunoreactivities were robust in all layers of the leptomeninges. Together with the previously reported expression of some neuronal and astroglial markers in cultured human leptomeninges, these observations suggest that culture alters the properties of leptomeningeal cells.
在重复研究中已证实，培养的人软脑膜（蛛网膜和软膜）细胞可表达典型的神经元蛋白，如神经丝蛋白和神经元特异性烯醇化酶。此外，它们还能表达胶质纤维酸性蛋白。本研究探讨了大鼠和人软脑膜细胞原位可能存在神经元和胶质蛋白的可能性。然而，160 kDa和200 kDa神经丝、神经元特异性烯醇化酶和微管相关蛋白2等神经元蛋白在软膜或蛛网膜中均未检测到免疫定位。胶质纤维酸性蛋白和半乳糖脑苷脂也未检测到，而纤连蛋白和波形蛋白的免疫反应性在软脑膜的所有层中均较强。连同先前报道的培养人软脑膜中一些神经元和星形胶质细胞标志物的表达，这些观察结果表明培养会改变软脑膜细胞的特性。
