Gulyaeva N V, Onufriev M V, Lazareva N A, Zarzhetsky Y V, Gurvitch A M, Volkov A V
Laboratory of Functional Biochemistry of the Nervous System, Russian Academy of Sciences, Moscow, Russia.
Neurosci Lett. 1996 Dec 20;220(3):147-50. doi: 10.1016/s0304-3940(96)13250-x.
Rats were subjected to 15 min cardiac arrest and sacrificed 1 h or 15-20 days after resuscitation. Homogenates of brain regions were assayed for nitric oxide synthase (NOS) activity (by measuring the mononitrosyl iron complex of NO with diethyl dithiocarbamate and endogenous brain Fe2+ using electron spin resonance spectroscopy) and generation of free radicals (FRG; by measuring H2O2-induced, luminol-dependent chemiluminescence). Cardiac arrest induced marked decrease of NOS activity and the increase of FRG, most prominent in cerebellum and less marked in cerebral cortex. Two groups of rats were revealed 15-20 days after cardiac arrest: with NOS activity significantly lower than control and not different from control. Positive linear inter-regional cross-correlations of both NOS activity and FRG (except of the group 1 h after resuscitation) as well as negative correlations between NOS and FRG were demonstrated.
将大鼠进行15分钟心脏骤停处理，并在复苏后1小时或15 - 20天处死。检测脑区匀浆中的一氧化氮合酶（NOS）活性（通过电子自旋共振光谱法测量NO与二乙基二硫代氨基甲酸盐和内源性脑Fe2+形成的单亚硝基铁络合物）以及自由基生成（FRG；通过测量H2O2诱导的、鲁米诺依赖性化学发光）。心脏骤停导致NOS活性显著降低和FRG增加，在小脑最为明显，在大脑皮层则不太明显。心脏骤停后15 - 20天发现两组大鼠：一组NOS活性显著低于对照组，另一组与对照组无差异。除复苏后1小时的组外，NOS活性和FRG均呈现区域间正线性交叉相关性，且NOS与FRG之间存在负相关性。
