Onufriev M V, Lazareva N A, Zarzhetsky Y V, Mutuskina E A, Gurvitch A M, Gulyaeva N V
Laboratory of Functional Biochemistry of Nervous System, Institute of Higher Nervous Activity and Neurophysiology, Russian Academy of Sciences, Moscow.
Neurochem Res. 1997 Jun;22(6):743-52. doi: 10.1023/a:1027314429219.
Effects of 7-min cardiac arrest and individual behavior on free radical-mediated processes and nitric oxide synthase (NOS) activity was evaluated in brains of male Wistar rats one hour and one week after resuscitation. "Emotional resonance" test was used for the behavioral selection of rats. The test includes factors of significance for rats: the choice between large and lighted or small and dark space as well as signals of pain of another rat. Free radical generation (using chemiluminescence method), superoxide scavenging/generating activity, substances reacting with 2-thiobarbituric acid and NOS activity (by measuring mononitrosyl iron complex of NO with diethyl dithiocarbamate and endogenous brain Fe2+ by electron spin resonance spectroscopy) were determined in cerebral cortex, cerebellum and hippocampus. Cardiac arrest induced oxidative stress accompanied by the loss of NOS activity, as well as compensatory changes of free radical-mediated processes in cerebral cortex. Oxidative stress was also evident in cerebellum and, to a lesser extent, in hippocampus. Most of neurochemical differences between behavioral groups were induced by cardiac arrest. These differences were global, related to a specific brain region or became apparent in cerebral lateralization of biochemical indices.
在雄性Wistar大鼠复苏后1小时和1周时，评估7分钟心脏骤停及个体行为对自由基介导过程和一氧化氮合酶（NOS）活性的影响。采用“情感共鸣”测试对大鼠进行行为筛选。该测试包括对大鼠具有重要意义的因素：在大而明亮或小而黑暗的空间之间进行选择，以及另一只大鼠的疼痛信号。在大脑皮层、小脑和海马中测定自由基生成（采用化学发光法）、超氧化物清除/生成活性、与2-硫代巴比妥酸反应的物质以及NOS活性（通过电子自旋共振光谱法测量NO与二乙基二硫代氨基甲酸盐的单亚硝基铁络合物和脑内源性Fe2+）。心脏骤停诱导氧化应激，伴有NOS活性丧失以及大脑皮层中自由基介导过程的代偿性变化。小脑也存在氧化应激，海马中的氧化应激程度较轻。行为组之间的大多数神经化学差异是由心脏骤停引起的。这些差异是全局性的，与特定脑区相关，或在生化指标的大脑侧化中变得明显。
