Smagin G N, Harris R B, Ryan D H
Laboratory of Neurosciences, Pennington Biomedical Research Center, Louisiana State University, Baton Rouge 70808, USA.
Neurosci Lett. 1996 Dec 20;220(3):167-70. doi: 10.1016/s0304-3940(96)13254-7.
It has been proposed that corticotropin-releasing factor (CRF) released during stress in the region of the locus coeruleus (LC) induces changes in behavior that are typical indices of anxiety. The experiments tested the ability of a CRF antagonist, alpha hCRF9-41, to attenuate stress-induced defensive withdrawal in rats. 1 microgram of alpha hCRF in 300 nl was infused bilaterally in the LC of rats 10 min prior to 30 min immobilization. The apparatus consisted of a small chamber set on one side of a 1 m open field, into which the rat was placed to start the test. Restraint induced defensive withdrawal in rats familiar with the apparatus and significantly increased latency time to emerge from the chamber, total time and mean time spent in the chamber. Infusion of alpha hCRF into the LC prior to restraint significantly decreased total and mean time spent in the chamber comparing to stressed animals. These results are consistent with anatomical, electrophysiological and neurochemical evidence that CRF receptors located in, or close to, the LC region influence behaviors induced by stress.
有人提出，在应激过程中蓝斑（LC）区域释放的促肾上腺皮质激素释放因子（CRF）会引发行为变化，这些变化是焦虑的典型指标。实验测试了CRF拮抗剂α hCRF9 - 41减轻大鼠应激诱导的防御性退缩的能力。在对大鼠进行30分钟固定前10分钟，将300纳升含1微克α hCRF的溶液双侧注入大鼠的蓝斑。实验装置由一个设置在1米开阔场地一侧的小室组成，将大鼠放入小室开始测试。束缚会诱导熟悉该装置的大鼠出现防御性退缩，并显著增加从室中出来的潜伏期、在室中花费的总时间和平均时间。与应激动物相比，在束缚前将α hCRF注入蓝斑显著减少了在室中花费的总时间和平均时间。这些结果与解剖学、电生理学和神经化学证据一致，即位于蓝斑区域或其附近的CRF受体影响应激诱导的行为。
