Demeler B, Saber H, Hansen J C
Department of Biochemistry, University of Texas Health Sciences Center at San Antonio 78284-7760, USA.
Biophys J. 1997 Jan;72(1):397-407. doi: 10.1016/S0006-3495(97)78680-6.
Synthetic sedimentation velocity boundaries were generated using finite-element solutions to the original and modified forms of the Lamm equation. Situations modeled included ideal single- and multicomponent samples, concentration-dependent samples, noninteracting multicomponent samples, and reversibly self-associating samples. Synthetic boundaries subsequently were analyzed using the method of van Holde and Weischet, and results were compared against known input parameters. Results indicate that this analytical method provides rigorous diagnostics for virtually every type of sample complexity encountered experimentally. Accordingly, both the power and utility of sedimentation velocity experiments have been significantly expanded.
利用对原始形式和修正形式的拉姆方程的有限元解生成了合成沉降速度边界。模拟的情况包括理想的单组分和多组分样品、浓度依赖性样品、非相互作用多组分样品以及可逆自缔合样品。随后使用范霍尔德和魏谢特方法分析合成边界，并将结果与已知输入参数进行比较。结果表明，这种分析方法为实验中遇到的几乎每种类型的样品复杂性提供了严格的诊断。因此，沉降速度实验的能力和实用性都得到了显著扩展。
