意大利成人和儿童粪便样本中脆弱拟杆菌肠毒素的检测及其毒素检测
Detection of enterotoxigenic Bacteroides fragilis and its toxin in stool samples from adults and children in Italy.
作者信息
Pantosti A, Menozzi M G, Frate A, Sanfilippo L, D'Ambrosio F, Malpeli M
机构信息
Bacteriology and Medical Mycology Laboratory, Istituto Superiore di Sanità, Rome, Italy.
出版信息
Clin Infect Dis. 1997 Jan;24(1):12-6. doi: 10.1093/clinids/24.1.12.
Stool samples from children and adults with and without diarrhea were examined for the presence of enterotoxigenic Bacteroides fragilis (ETBF) and its enterotoxin. A cytotoxic assay with HT-29 cells followed by neutralization with a hyperimmune antiserum were used to detect B. fragilis enterotoxin. ETBF isolates were recovered from 12% of healthy children and 17% of children with diarrhea (P = .42) and from 15% of healthy adults and 9.4% of adults with diarrhea (P = .31). Fecal B. fragilis enterotoxin was detected in four children (two with diarrhea and two without diarrhea) and in four adults with diarrhea. This study shows that in Italy, the rate of ETBF carriage is high, regardless if diarrhea is present. In some instances, the presence of ETBF is associated with detectable levels of fecal enterotoxin, but the significance of this finding deserves further evaluation.
对有腹泻和无腹泻的儿童及成人的粪便样本进行检测，以确定是否存在产肠毒素脆弱拟杆菌（ETBF）及其肠毒素。采用HT - 29细胞进行细胞毒性试验，随后用超免疫抗血清进行中和，以检测脆弱拟杆菌肠毒素。从12%的健康儿童、17%的腹泻儿童（P = 0.42）、15%的健康成人和9.4%的腹泻成人中分离出ETBF（P = 0.31）。在4名儿童（2名腹泻儿童和2名无腹泻儿童）和4名腹泻成人中检测到粪便脆弱拟杆菌肠毒素。这项研究表明，在意大利，无论是否存在腹泻，ETBF携带率都很高。在某些情况下，ETBF的存在与粪便肠毒素的可检测水平相关，但这一发现的意义值得进一步评估。
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