Guo H, Zucker S, Gordon M K, Toole B P, Biswas C
Department of Anatomy and Cellular Biology, Tufts University School of Medicine, Boston, Massachusetts 02111, USA.
J Biol Chem. 1997 Jan 3;272(1):24-7.
Many of the tumor-associated matrix metalloproteinases that are implicated in metastasis are produced by stromal fibroblasts within or surrounding the tumor in response to stimulation by factors produced by tumor cells. In this study we transfected Chinese hamster ovary cells with putative cDNA for human extracellular matrix metalloproteinase inducer (EMMPRIN), a transmembrane glycoprotein that is attached to the surface of many types of malignant human tumor cells and that has previously been implicated in stimulation of matrix metalloproteinase production in fibroblasts. We show that these transfected cells synthesize EMMPRIN that is extensively post-translationally processed; this recombinant EMMPRIN stimulates human fibroblast production of interstitial collagenase, stromelysin-1, and gelatinase A (72-kDa type IV collagenase). We propose that EMMPRIN regulates matrix metalloproteinase production during tumor invasion and other processes involving tissue remodeling.
许多与转移相关的肿瘤相关基质金属蛋白酶是由肿瘤内部或周围的基质成纤维细胞产生的，以响应肿瘤细胞产生的因子的刺激。在本研究中，我们用人类细胞外基质金属蛋白酶诱导剂（EMMPRIN）的推定cDNA转染中国仓鼠卵巢细胞，EMMPRIN是一种跨膜糖蛋白，附着于多种类型的人类恶性肿瘤细胞表面，此前已被证明与刺激成纤维细胞产生基质金属蛋白酶有关。我们发现这些转染细胞合成了经过广泛翻译后加工的EMMPRIN；这种重组EMMPRIN刺激人类成纤维细胞产生间质胶原酶、基质溶解素-1和明胶酶A（72 kDa IV型胶原酶）。我们提出，EMMPRIN在肿瘤侵袭和其他涉及组织重塑的过程中调节基质金属蛋白酶的产生。
