Saïb A, Puvion-Dutilleul F, Schmid M, Périès J, de Thé H
CNRS UPR 9051, Hôpital Saint-Louis, Paris, France.
J Virol. 1997 Feb;71(2):1155-61. doi: 10.1128/JVI.71.2.1155-1161.1997.
The pathways used in the transport of retroviral genomes to the nucleus are poorly identified. Analyzing the intracellular localization of incoming foamy viruses, we have found that the Gag antigens and the viral genome accumulate in a distinct perinuclear domain identified as the centrosome. Colchicine treatment completely abolished pericentriolar targeting of human foamy virus (HFV) proteins, suggesting a role for microtubules in the transport of the incoming viral proteins to the centrioles. Finally, we demonstrate that, similarly to human immunodeficiency virus DNA, HFV DNA can enter the nucleus of G1/S-phase-arrested cells, although no viral gene expression can be observed. Recent observations have demonstrated that foamy viruses have several features not shared by other retroviruses. The intracellular route of the incoming Gag antigens may constitute a new specificity of this class of viruses.
逆转录病毒基因组向细胞核运输所使用的途径目前还不太明确。通过分析传入的泡沫病毒在细胞内的定位，我们发现Gag抗原和病毒基因组聚集在一个被确定为中心体的独特核周区域。秋水仙碱处理完全消除了人类泡沫病毒（HFV）蛋白向中心粒周围的靶向定位，这表明微管在将传入的病毒蛋白运输到中心粒过程中发挥了作用。最后，我们证明，与人类免疫缺陷病毒DNA类似，HFV DNA可以进入G1/S期阻滞细胞的细胞核，尽管未观察到病毒基因表达。最近的观察结果表明，泡沫病毒具有其他逆转录病毒所没有的几个特征。传入的Gag抗原的细胞内途径可能构成了这类病毒的一种新特性。
