Contag C H, Ehrnst A, Duda J, Bohlin A B, Lindgren S, Learn G H, Mullins J I
Department of Pediatrics, Stanford University School of Medicine, California, USA.
J Virol. 1997 Feb;71(2):1292-300. doi: 10.1128/JVI.71.2.1292-1300.1997.
Genetic analysis of human immunodeficiency virus type 1 (HIV-1) from cases of mother-to-infant transmission were analyzed in an effort to provide insights into the viral selection that may occur during transmission, as well as the timing and source of transmitted viruses. HIV-1 env genes obtained from seven mothers and their perinatally infected infants in Sweden were studied. Five envelope sequence clades (A to E) were found to be represented. We used a heteroduplex tracking assay (HTA) to assess the genetic relatedness between early viral isolates from the infants and serial maternal virus populations taken during pregnancy and at delivery. HTA findings were used to select for DNA sequence analysis maternal virus populations that were either closely or more distantly related to the infant virus. In each case, nucleotide sequence analysis confirmed the genetic relationships inferred by the HTA. Only maternal peripheral blood was sampled, and large sets of maternal specimens throughout pregnancy were generally not available. However, no consistent correlation was found to support the hypothesis that infant viruses should match blood-derived maternal virus genotypes found early in pregnancy if infants were found to be infected at birth or, conversely, that infant viruses should match blood-derived maternal virus genotypes found at delivery if infants were found to be infected only some time later.
对母婴传播病例中的1型人类免疫缺陷病毒（HIV-1）进行了基因分析，旨在深入了解传播过程中可能发生的病毒选择情况，以及传播病毒的时间和来源。研究了从瑞典的7名母亲及其围产期感染婴儿身上获得的HIV-1 env基因。发现有五个包膜序列分支（A至E）。我们使用异源双链追踪分析（HTA）来评估婴儿早期病毒分离株与孕期和分娩时采集的母亲系列病毒群体之间的遗传相关性。HTA结果用于选择与婴儿病毒密切或较远相关的母亲病毒群体进行DNA序列分析。在每种情况下，核苷酸序列分析都证实了HTA推断的遗传关系。仅采集了母亲的外周血，整个孕期的大量母亲样本通常无法获得。然而，未发现一致的相关性来支持以下假设：如果婴儿在出生时被发现感染，那么婴儿病毒应与孕期早期发现的血液来源的母亲病毒基因型匹配；相反，如果婴儿仅在一段时间后被发现感染，那么婴儿病毒应与分娩时发现的血液来源的母亲病毒基因型匹配。
