Pasquier C, Cayrou C, Blancher A, Tourne-Petheil C, Berrebi A, Tricoire J, Puel J, Izopet J
Laboratoire de Virologie, Centre Hospitalier Universitaire Purpan, Toulouse, France.
J Virol. 1998 Nov;72(11):8493-501. doi: 10.1128/JVI.72.11.8493-8501.1998.
We have examined the viral selection that may occur during transmission by studying the env gene sequences from four cases of mother-to-child transmission of human immunodeficiency virus type 1. The V3 region sequences were directly amplified from both plasma viral RNA and peripheral blood mononuclear cells containing proviral DNA from mothers at delivery and at the time of diagnosis for children. Transmission occurred perinatally in three cases. The similarity of the viral sequences in each infant sample contrasted with the heterogeneous viral populations in the mothers. Phylogenetic analysis indicated the transmission of one or a few closely related maternal minor virus variants. In contrast, the child virus population in the fourth case was as heterogeneous as that of his mother, and phylogenetic analysis strongly suggested the transmission of multiple maternal variants. This case of multiple transmission was confirmed by analyzing sequences obtained at three times after delivery. Strains with sequences corresponding to the syncytium-inducing phenotype were also transmitted in this fourth case, and this was associated with the rapid development of disease in the child. There was no evidence for transmission of particular viral variants from mother to infant. We have thus described a particular case of vertical human immunodeficiency virus type 1 transmission with the transmission of multiple maternal variants to the infant and a rapid, fatal outcome in the child.
我们通过研究4例人类免疫缺陷病毒1型母婴传播病例的env基因序列，检测了传播过程中可能发生的病毒选择情况。V3区序列直接从分娩时母亲的血浆病毒RNA以及含有前病毒DNA的外周血单核细胞中扩增得到，同时也从儿童诊断时的样本中进行扩增。3例为围产期传播。每个婴儿样本中病毒序列的相似性与母亲体内病毒群体的异质性形成对比。系统发育分析表明，有一个或几个密切相关的母亲体内的次要病毒变体发生了传播。相比之下，第四例中儿童的病毒群体与母亲的一样具有异质性，系统发育分析强烈提示有多个母亲体内的变体发生了传播。通过分析分娩后三个时间点获得的序列，证实了这例多变体传播的情况。在第四例中，具有与合胞体诱导表型相对应序列的毒株也发生了传播，这与儿童疾病的快速发展有关。没有证据表明特定的病毒变体从母亲传播给了婴儿。因此，我们描述了一例特殊的人类免疫缺陷病毒1型垂直传播病例，即多个母亲体内的变体传播给了婴儿，并导致儿童迅速死亡。
