Liu Y J, Xu J, de Bouteiller O, Parham C L, Grouard G, Djossou O, de Saint-Vis B, Lebecque S, Banchereau J, Moore K W
Schering-Plough, Laboratory for Immunological Research, Dardilly, France.
J Exp Med. 1997 Jan 6;185(1):165-70. doi: 10.1084/jem.185.1.165.
This paper describes an antibody (mAb 7D6) that specifically recognizes human follicular dendritic cells (FDCs). By expression cloning, a cDNA clone encoding for the long human CR2/ CD21 isoform (CD21L) that contains an additional exon (10a) was isolated. We demonstrated that FDCs selectively express CD21L, while B cells selectively express the short CR2/CD21 lacking exon 10a (CD21S). By screening mouse Ltk- cells transfected with the CD21L cDNA, we further showed that the other two anti-human FDC mAbs DRC-1 and KiM4 also recognize CD21L. Thus, CD21L represents the first characterized human FDC-specific molecule, which may confer unique functions of FDCs in germinal center development.
本文描述了一种特异性识别人类滤泡树突状细胞（FDCs）的抗体（单克隆抗体7D6）。通过表达克隆，分离出了一个编码包含额外外显子（10a）的长人类CR2/CD21同种型（CD21L）的cDNA克隆。我们证明FDCs选择性表达CD21L，而B细胞选择性表达缺乏外显子10a的短CR2/CD21（CD21S）。通过筛选用CD21L cDNA转染的小鼠Ltk-细胞，我们进一步表明另外两种抗人类FDC单克隆抗体DRC-1和KiM4也识别CD21L。因此，CD21L代表了首个被鉴定的人类FDC特异性分子，其可能赋予FDCs在生发中心发育中的独特功能。
