Orós J, Matsushita S, Rodríguez J L, Rodríguez F, Fernández A
Department of Histology and Pathology, Veterinary Faculty of Las Palmas, Canary Islands, Spain.
J Vet Med Sci. 1996 Dec;58(12):1219-21. doi: 10.1292/jvms.58.12_1219.
Immunohistochemical detection of rat CAR bacillus antigen in paraffin-embedded experimentally infected rat lungs, using an immunoperoxidase technique based on the labelled streptavidin biotin (LSAB) method and 3-amino-9-ethylcarbazole (AEC) as substrate is described in this paper. The pattern of immunostaining was confined to the ciliated bronchial epithelium and the specificity of this technique was confirmed. The use of AEC as substrate was evaluated more efficient than diaminobenzidine (DAB). The usefulness of this immunoperoxidase technique for the detection of CAR bacillus in rats and its advantages compared to the indirect immunofluorescence (IF) are discussed.
本文描述了使用基于标记链霉亲和素生物素（LSAB）法和以3-氨基-9-乙基咔唑（AEC）为底物的免疫过氧化物酶技术，对石蜡包埋的经实验感染的大鼠肺组织中的大鼠CAR杆菌抗原进行免疫组织化学检测。免疫染色模式局限于纤毛支气管上皮，且该技术的特异性得到了证实。评估结果表明，使用AEC作为底物比二氨基联苯胺（DAB）更有效。讨论了这种免疫过氧化物酶技术在大鼠中检测CAR杆菌的实用性及其与间接免疫荧光（IF）相比的优势。
