Griffith J W, White W J, Danneman P J, Lang C M
Department of Comparative Medicine, Milton S. Hershey Medical Center, Pennsylvania State University, Hershey.
Vet Pathol. 1988 Jan;25(1):72-6. doi: 10.1177/030098588802500110.
Cilia-associated respiratory (CAR) bacillus was identified in respiratory tract lesions of obese mice dying of chronic respiratory disease. Neither Mycoplasma pulmonis nor pathogenic bacteria were isolated from cultures of the lesions at necropsy, but there was serologic and histologic evidence of respiratory virus infection. Cranial-ventral areas of lung were firm and demarcated from unaffected lung at gross examination, and representative tissue sank in water. Microscopically, there was suppurative bronchopneumonia with extensive peribronchiole lymphocyte and plasma cell proliferation. The affected bronchiole epithelium was covered with a sheet of slightly basophilic, filamentous, gram negative bacteria. Bronchioles with lesser amounts of lymphocyte accumulations contained lesser amounts of filamentous bacteria. Bronchioles without filamentous bacteria lining the respiratory epithelium lacked peribronchiole lymphocyte accumulations. There was a high correlation between CAR bacillus-positive serology and the identification of diagnostic histologic lesions. CAR bacillus was readily stained using immunohistochemical methods, and the ultrastructural features were similar to that described in rat infections.
在死于慢性呼吸道疾病的肥胖小鼠的呼吸道病变中发现了纤毛相关呼吸道（CAR）杆菌。尸检时，在病变培养物中未分离到肺支原体或病原菌，但有呼吸道病毒感染的血清学和组织学证据。大体检查时，肺的颅腹侧区域质地坚实，与未受影响的肺分界明显，代表性组织在水中下沉。显微镜下，有化脓性支气管肺炎，伴有广泛的细支气管周围淋巴细胞和浆细胞增殖。受影响的细支气管上皮覆盖着一层轻度嗜碱性、丝状、革兰氏阴性细菌。淋巴细胞聚集较少的细支气管中丝状细菌数量也较少。呼吸道上皮没有丝状细菌衬里的细支气管缺乏细支气管周围淋巴细胞聚集。CAR杆菌阳性血清学与诊断性组织学病变的鉴定之间存在高度相关性。使用免疫组织化学方法很容易对CAR杆菌进行染色，其超微结构特征与大鼠感染中描述的相似。
