Pérez F J, Navarro D, Gimeno C, García-de-Lomas J
Department of Microbiology, School of Medicine, University Hospital, Valencia, Spain.
Chemotherapy. 1997 Jan-Feb;43(1):27-30. doi: 10.1159/000239531.
Previously, it has been postulated that the porin OprC facilitates the diffusion of ceftazidime through the outer membrane of Pseudomonas aeruginosa. To further investigate this claim, the outer membrane protein (OMP) profiles of 22 ceftazidime-susceptible clinical isolates were analyzed. No correlation was found between MIC values and the level of expression of OprC. Further, OprC was either undetectable or expressed in reduced amounts in 12 isolates. In contrast, OprF and OprE were present in all isolates studied. This study suggests that OprC is dispensable for the permeation of ceftazidime through the outer membrane of P. aeruginosa.
此前曾有人推测，孔蛋白OprC有助于头孢他啶通过铜绿假单胞菌的外膜扩散。为了进一步研究这一说法，分析了22株对头孢他啶敏感的临床分离株的外膜蛋白（OMP）谱。未发现最低抑菌浓度（MIC）值与OprC表达水平之间存在相关性。此外，在12株分离株中，要么检测不到OprC，要么其表达量降低。相比之下，在所研究的所有分离株中都存在OprF和OprE。这项研究表明，OprC对于头孢他啶透过铜绿假单胞菌外膜的渗透并非必需。
