Pérez F J, Gimeno C, Navarro D, García-de-Lomas J
Department of Microbiology, University Hospital, Valencia, Spain.
Chemotherapy. 1996 May-Jun;42(3):210-14. doi: 10.1159/000239444.
The outer membrane protein (OMP) OprD is the major channel through which carbapenems permeate the outer membrane of Pseudomonas aeruginosa. In this study, we analyzed the OMP profiles of several P. aeruginosa clinical isolates showing diminished susceptibility to imipenem while remaining susceptible to meropenem. All these isolates lacked OprD or showed a reduced expression of this porin. Susceptibility to meropenem was thus independent of the level of OprD expression, indicating that the antimicrobial could be taken up via an alternative route. The level of expression of OprC (70 kD) was also unrelated to meropenem susceptibility. Nevertheless, OMPs OprF and OprE were expressed by all isolates, suggesting that in the absence of OprD, these porins might be involved in the permeation of meropenem.
外膜蛋白（OMP）OprD是碳青霉烯类药物透过铜绿假单胞菌外膜的主要通道。在本研究中，我们分析了几株对亚胺培南敏感性降低但对美罗培南仍敏感的铜绿假单胞菌临床分离株的OMP谱。所有这些分离株均缺乏OprD或该孔蛋白的表达降低。因此，对美罗培南的敏感性与OprD表达水平无关，这表明该抗菌药物可通过替代途径摄取。OprC（70 kD）的表达水平也与美罗培南敏感性无关。然而，所有分离株均表达OMPs OprF和OprE，这表明在缺乏OprD的情况下，这些孔蛋白可能参与美罗培南的渗透。
