Ruiz-Montasell B, Aguado F, Majó G, Chapman E R, Canals J M, Marsal J, Blasi J
Departament de Biologia Cellular i Anatomia Patològica, Facultat de Medicina, Universitat de Barcelona, Spain.
Eur J Neurosci. 1996 Dec;8(12):2544-52. doi: 10.1111/j.1460-9568.1996.tb01548.x.
Syntaxin 1 binds to several proteins of the synaptic terminal and is a central component in the pathway of protein-protein interactions that underlies docking and fusion of synaptic vesicles. Molecular studies revealed the occurrence of two isoforms, syntaxin 1A and syntaxin 1B, which coexpress in neural tissues. However, they display differential expression patterns in endocrine cell types. We generated isoform-specific antibodies that were used in Western blotting and immunocytochemical studies. First, we confirmed the sole presence of syntaxin 1A in endocrine pituitary cells. Second, we found distinctive immunolabelling patterns of each isoform in the rat olfactory system, hippocampus, striatum, thalamus and spinal cord. In addition, the principal white matter commissures displayed distinct immunoreactivity for each isoform. This report shows, for the first time, major differences between the distributions of syntaxin 1A and syntaxin 1B isoforms in the rat central nervous system.
Syntaxin 1与突触终末的几种蛋白质结合，是介导突触小泡停靠和融合的蛋白质-蛋白质相互作用途径中的核心成分。分子研究显示存在两种同工型，即Syntaxin 1A和Syntaxin 1B，它们在神经组织中共表达。然而，它们在内分泌细胞类型中表现出不同的表达模式。我们制备了同工型特异性抗体，并将其用于蛋白质印迹法和免疫细胞化学研究。首先，我们证实内分泌垂体细胞中仅存在Syntaxin 1A。其次，我们在大鼠嗅觉系统、海马体、纹状体、丘脑和脊髓中发现了每种同工型独特的免疫标记模式。此外，主要的白质连合对每种同工型表现出不同的免疫反应性。本报告首次展示了大鼠中枢神经系统中Syntaxin 1A和Syntaxin 1B同工型分布的主要差异。
