通过毛细管电泳对生物相关有机酸进行间接紫外检测。
Indirect ultraviolet detection of biologically relevant organic acids by capillary electrophoresis.
作者信息
Chen H, Xu Y, Van Lente F, Ip M P
机构信息
Department of Chemistry, Cleveland State University, OH 44115, USA.
出版信息
J Chromatogr B Biomed Appl. 1996 Apr 26;679(1-2):49-59. doi: 10.1016/0378-4347(96)00002-3.
Indirect UV detection of fourteen short-chain organic acids (e.g., oxalic acid, citric acid, malonic acid, tartaric acid, methylmalonic acid, alpha-ketoglutaric acid, succinic acid, ethylmalonic acid, methylsuccinic acid, glutaric acid, apidic acid, methylglutaric acid, lactic acid and pyruvic acid) by capillary electrophoresis is described. The method used phthalate as the UV-absorbing additive in carbonate buffer and the non-absorbing analytes were detected indirectly at 230 nm. The influences of buffer pH, ionic strength, concentration of phthalate and organic modifier on indirect signal response and migration behavior of the organic acids were investigated. Comparisons of reproducibility on migration time, limit of detection and separation efficiency among three types of capillary (e.g., polyacrylamide-coated, myristyltrimethylammonium bromide-coated and uncoated capillaries) were conducted. The method developed was applied to detect succinic acid, methylmalonic acid, citric acid, glutaric acid and lactic acid in human body fluids, and preliminary results were provided.
描述了通过毛细管电泳间接紫外检测十四种短链有机酸（例如草酸、柠檬酸、丙二酸、酒石酸、甲基丙二酸、α-酮戊二酸、琥珀酸、乙基丙二酸、甲基琥珀酸、戊二酸、阿地酸、甲基戊二酸、乳酸和丙酮酸）的方法。该方法使用邻苯二甲酸盐作为碳酸盐缓冲液中的紫外吸收添加剂，在230nm处间接检测无吸收的分析物。研究了缓冲液pH值、离子强度、邻苯二甲酸盐浓度和有机改性剂对有机酸间接信号响应和迁移行为的影响。对三种类型的毛细管（例如聚丙烯酰胺涂层毛细管、十四烷基三甲基溴化铵涂层毛细管和未涂层毛细管）的迁移时间重现性、检测限和分离效率进行了比较。所开发的方法应用于检测人体体液中的琥珀酸、甲基丙二酸、柠檬酸、戊二酸和乳酸，并给出了初步结果。
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