Majtán V, Majtánová L
Institute of Preventive and Clinical Medicine, Bratislava, Slovak Republic.
Pharmazie. 1996 Jan;51(1):54-6.
The effect of a homologous series of 2-(dodecanoylamino)-ethylalkyl-dimethylammonium bromides on the cell metabolism of a Pseudomonas aeruginosa strain isolated from a nosocomial infection was studied. The inhibition of the incorporation of radioactive precursors into macromolecules (DNA, RNA, proteins) was dependent on the length of the alkyl chain of the tested substances. Similar results were noted also by following the inhibition of the endogenous respiration of P. aeruginosa. The most effective substance at suppression of [14C] precursors incorporation was with hexyl, and in the case of respiration, the substance with octyl in the molecule. The total inhibition of the respiration in the presence of exogenous carbon substrates, i.e. glucose and glycerol, was found with the substance containing the octyl group (conc. 100 micrograms/ml). We assumed that the studied substances interfere with cell energy-carbon metabolism of P. aeruginosa.
研究了一系列2-(十二烷酰氨基)-乙基烷基二甲基溴化铵对从医院感染中分离出的铜绿假单胞菌菌株细胞代谢的影响。放射性前体掺入大分子（DNA、RNA、蛋白质）的抑制作用取决于受试物质烷基链的长度。通过跟踪铜绿假单胞菌内源性呼吸的抑制情况也得到了类似结果。抑制[14C]前体掺入最有效的物质是含己基的物质，而在呼吸抑制方面，分子中含辛基的物质效果最佳。在存在外源碳底物（即葡萄糖和甘油）的情况下，发现含辛基的物质（浓度为100微克/毫升）能完全抑制呼吸作用。我们推测所研究的物质会干扰铜绿假单胞菌的细胞能量-碳代谢。
