Sokolova T M, Uryvaev L V, Selivanova T K, Bobrova O V, Lebedev A Iu, Bystrov N S
Vopr Virusol. 1996 Nov-Dec;41(6):245-52.
A recombination of the genomes of VEE and KF viruses, two alphaviruses of different serotypes, takes place in mixed infection of chick embryo fibroblasts and Vero cells with vaccine strain VEE-230 and KF-9298 strain. This recombination resulted in the production of recombinant virions with altered antigenic properties. Polymerase chain reaction (PCR) rapidly and effectively detected the hybrid sites of the genome in recombinant viruses. Short nucleotide sequences (approximately 20 n. p.) of a known primary structure in the RNA genomes, specifically reacting with the complementary PCR primers, were chosen as genetic markers. These data are the first experimental validation of the probable recombinant formation of alphaviruses in nature. They are in good agreement with findings of the recombinant structure of Western equine encephalomyelitis virus genome.
委内瑞拉马脑炎（VEE）病毒和基孔肯雅热（KF）病毒是两种不同血清型的甲病毒，当用疫苗株VEE - 230和KF - 9298株对鸡胚成纤维细胞和非洲绿猴肾细胞进行混合感染时，这两种病毒的基因组会发生重组。这种重组导致产生了具有改变抗原特性的重组病毒粒子。聚合酶链反应（PCR）能够快速有效地检测重组病毒基因组中的杂交位点。RNA基因组中已知一级结构的短核苷酸序列（约20个核苷酸），因其能与互补的PCR引物特异性反应，被选作遗传标记。这些数据首次通过实验验证了甲病毒在自然环境中可能形成重组体。它们与西方马脑炎病毒基因组的重组结构研究结果高度吻合。
