Berquist K R, Maynard J E, Francy D B, Sheller M G, Schable C A
Am J Trop Med Hyg. 1976 Sep;25(5):730-2. doi: 10.4269/ajtmh.1976.25.730.
Culex tarsalis and Aedes aegypti mosquitoes were fed on chimpanzees carrying hepatitis B surface antigen (HBS Ag) of known infectivity and pools were tested by radioimmunoassay daily for the presence of HBS Ag. HBS Ag continued to be detected at low levels in mosquito tissue after digestion of the blood meal. Inoculation of susceptible chimpanzees with macerated pools of A. aegypti mosquitoes at two intervals after digestion of the blood meal did not produce hepatitis or serologic evidence of hepatitis B virus infection. Mechanical transmission studies by interrupting feeding of A aegypti from HBS Ag-carrier chimpanzees and transferring them to susceptible chimpanzees did not produce hepatitis. These findings do not support the hypothesis that mosquitoes are involved in either biological or mechanical transmission of hepatitis B.
将致倦库蚊和埃及伊蚊喂养于携带已知传染性乙肝表面抗原（HBS Ag）的黑猩猩，每天通过放射免疫分析法检测蚊群中是否存在HBS Ag。在血餐消化后，仍可在蚊组织中持续检测到低水平的HBS Ag。在血餐消化后的两个时间点，用研磨后的埃及伊蚊蚊群接种易感黑猩猩，未引发肝炎或出现乙肝病毒感染的血清学证据。通过中断埃及伊蚊从携带HBS Ag的黑猩猩处取食并将其转移至易感黑猩猩进行机械传播研究，未引发肝炎。这些发现不支持蚊子参与乙肝生物传播或机械传播这一假说。
