Costello P S, Turner M, Walters A E, Cunningham C N, Bauer P H, Downward J, Tybulewicz V L
National Institute for Medical Research, London, UK.
Oncogene. 1996 Dec 19;13(12):2595-605.
Activation of the high affinity IgE receptor (Fc epsilon RI) of mast cells, a member of the antigen receptor family, leads to the release of allergic mediators, a critical event in the onset of immediate hypersensitivity. Stimulation of Fc epsilon RI results in the rapid association and activation of the Syk tyrosine kinase. Using Syk-deficient mast cells we show that they fail to degranulate, synthesize leukotrienes and secrete cytokines when stimulated through Fc epsilon RI, conclusively demonstrating an essential role for Syk in Fc epsilon RI signalling. Furthermore, our data strongly supports a model of Fc epsilon RI engagement leading to the sequential activation of the tyrosine kinases Lyn and then Syk. A similar mechanism is likely to apply to signal transduction through all members of the antigen receptor family.
肥大细胞的高亲和力IgE受体（FcεRI）作为抗原受体家族的一员，其激活会导致过敏介质的释放，这是速发型超敏反应发生过程中的关键事件。FcεRI的刺激会导致Syk酪氨酸激酶迅速结合并激活。利用缺乏Syk的肥大细胞，我们发现当通过FcεRI刺激时，它们无法脱颗粒、合成白三烯和分泌细胞因子，这确凿地证明了Syk在FcεRI信号传导中起着至关重要的作用。此外，我们的数据有力地支持了一种模型，即FcεRI的结合会导致酪氨酸激酶Lyn先被激活，然后Syk被激活。类似的机制可能适用于通过抗原受体家族所有成员进行的信号转导。
